| Vegetation exists widely in river channels, which can effectively improve the water ecological environment and increase open channels' flow resistance. Usually, the stick of different materials or bionic plants is used to simulate actual plants for experimental research, and plant resistance is added to the bed surface to form equivalent bed surface resistance together with the original bed surface resistance. The study on equivalent bed resistance of watercourses containing plants is not perfect because of the dissimilarity of simulating plants and actual plants. In this paper, applying actual velocity conditions to existing plant zones was proposed. According to the plant density in the field investigation of the river, the river was divided into transverse and vertical sections, and the actual flow velocity conditions were applied to the actual plant areas. The plant resistance in the zoned water body was experimentally studied, and the equivalent bed roughness representing the equivalent bed resistance was finally converted. Taking the upper reaches of Duliujian River as an example, three typical plants, namely arbors, shrubs and reeds, were selected to carry out experiments considering the velocity conditions of different zones in the cross-section of the river. The results showed that when the one-in-100-year flood occurs, the equivalent bed roughness of the arbor planting area is 0.060~0.066, and that of the shrub planting area is 0.083~0.099. The equivalent bed roughness in the reed planting area ranges from 0.698 to 0.989. The research results can provide theoretical support for flood control calculation and water environment treatment.